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Why caffeine as a reference standard used for calibration
of HPLC?
- 11 Answers
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Answer / milind patil
Because of
# It gives a multiwavelength response like 205,245,273 nm
# It is easily available in market
# It is very stable, we can use a long time for analysis
i.e. it is not degradable
# It is chip
| Is This Answer Correct ? | 74 Yes | 8 No |
Answer / abdulkalim
In general we all use caffeine as a standard for
calibration of HPLC specially for (Calibration of Wave
Length Accuracy) since it will scan the whole of the UV
range i.e
1. Maxima at 205 nm
2. Minima at 245 nm
3. Maxima at 273 nm
Since it is a universal known standard.
It is stable and easily available in the market.
But here comes the question how about the calibration of
wavelength accuracy at Visible range. In this case we can
use Erbium Perchlorate which will scan the whole range(200
nm to 600 nm).
It is stable and little costly but availability in the
market when compared to caffeine is litte tough.
| Is This Answer Correct ? | 39 Yes | 2 No |
Answer / yojana
it is use as primary standard .it gives two maxima & one
minima
first maxima at 205 nm
second maxima at 273 nm
&
minima at 245nm
also caffeine easily available in market.
| Is This Answer Correct ? | 37 Yes | 7 No |
Answer / latif ahmed [ozone pharma,guw
1.caffeine is available in anhydrous form.
2.Shows maxima at two different wavelength ie.205nm & 273nm
3.Cheap & easily available.
| Is This Answer Correct ? | 15 Yes | 2 No |
Answer / rohidas baban shinde
Because it shows maxima at the wavelength of 205 nm and 273
nm and shows and also shows minima at the wavelength of 245
nm. It is a Primary reference standard and it is avaliable
in the market very easily.It is cheap.It is non-degradable
and we can used it for longer period of analysis.
| Is This Answer Correct ? | 11 Yes | 0 No |
Answer / kalpesh
# it is used for wavelength accuracy for HPLC
# It gives a multiwavelength response like 205,245,273 nm
# 205nm maxima, 245nm minima, 273nm maxima
# It is easily available in market
# It is very stable, we can use a long time for analysis
i.e. it is not degradable
# It is chip
| Is This Answer Correct ? | 7 Yes | 0 No |
Answer / santosh
Addition to above it is unretained compound so no
possibility of carryover to the chromatographic system
| Is This Answer Correct ? | 8 Yes | 2 No |
Answer / srinivasarao yemineni
1. Maxima at 205 nm
2. Minima at 245 nm
3. Maxima at 273 nm
Since it is a universal known standard.
It is stable and easily available in the market.
But here comes the question how about the calibration of
wavelength accuracy at Visible range. In this case we can
use Erbium Perchlorate which will scan the whole range(200
nm to 600 nm).
It is stable and little costly but availability in the
market when compared to caffeine is litte tough.
Thanks for Marking this Answer
| Is This Answer Correct ? | 6 Yes | 1 No |
Answer / neelam tyagi
it shows maxima at the multi wavelength of 205 nm and 273
nm shows and shows minima at the wavelength of 245
nm. It is a Primary reference standard andvery easily
avaliable
in the market .It is cheap.It is non-degradable
and we can used it for longer period of analysis it is very
highly stable
| Is This Answer Correct ? | 2 Yes | 0 No |
Answer / irfan shaikh
Caffeine is secondary standard.
It is anhydrous and remain stable long time
| Is This Answer Correct ? | 1 Yes | 2 No |
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